Journal: Cells

Article Title: Distinct Infiltration of T Cell Populations in Bladder Cancer Molecular Subtypes

doi: 10.3390/cells13110926

Figure Lengend Snippet: Antibodies used for flow cytometry analysis and sorting of T cell populations from tumor and control bladder tissues.

Article Snippet: APC Mouse Anti-Human CD25 , M-A251 , BD biosciences.

Techniques: Flow Cytometry, Staining

Journal: STAR Protocols

Article Title: Protocol for isolation of tumor-derived extracellular vesicles and functional studies on human T cell subsets

doi: 10.1016/j.xpro.2024.103011

Figure Lengend Snippet: Gating strategy for FACS sorting of T cell subsets (A) Gating strategy for sorting of T cell subsets in presented example. First, lymphocytes are gated based on physical parameters (SSC-A, FSC-A) and single cells are identified by comparing forward scatter height and area (FSC-H vs. FSC-A). Then, CD4+ and CD8+ are identified, and among CD4+ T cells Tregs and non-Treg CD4+ cells (Tconv) are divided based on expression of CD25 and CD127. (B) Analysis of sorting purity for sorted CD8+ T cells (left), non-Treg CD4+/Tconv (middle & top right) and Tregs (middle & bottom right).

Article Snippet: Mouse monoclonal anti-human CD25 APC (cl. CD25-4E3) , eBioscience/Invitrogen , Cat# 17−0257−42; RRID: AB_11218671.

Techniques: Expressing

Journal: STAR Protocols

Article Title: Protocol for isolation of tumor-derived extracellular vesicles and functional studies on human T cell subsets

doi: 10.1016/j.xpro.2024.103011

Figure Lengend Snippet:

Article Snippet: Mouse monoclonal anti-human CD25 APC (cl. CD25-4E3) , eBioscience/Invitrogen , Cat# 17−0257−42; RRID: AB_11218671.

Techniques: Purification, Recombinant, Staining, Software, Cell Culture, Flow Cytometry

Journal: STAR Protocols

Article Title: Protocol for isolation of tumor-derived extracellular vesicles and functional studies on human T cell subsets

doi: 10.1016/j.xpro.2024.103011

Figure Lengend Snippet: Antibody mix for T cell sorting

Article Snippet: Mouse monoclonal anti-human CD25 APC (cl. CD25-4E3) , eBioscience/Invitrogen , Cat# 17−0257−42; RRID: AB_11218671.

Techniques: Concentration Assay

Journal: STAR Protocols

Article Title: Protocol for isolation of tumor-derived extracellular vesicles and functional studies on human T cell subsets

doi: 10.1016/j.xpro.2024.103011

Figure Lengend Snippet: Antibody mix for T cell phenotyping

Article Snippet: Mouse monoclonal anti-human CD25 APC (cl. CD25-4E3) , eBioscience/Invitrogen , Cat# 17−0257−42; RRID: AB_11218671.

Techniques: Concentration Assay, Staining

Journal: Journal of visualized experiments : JoVE

Article Title: Xenograft Skin Model to Manipulate Human Immune Responses In Vivo

doi: 10.3791/64040

Figure Lengend Snippet: Materials

Article Snippet: APC mouse anti-human CD25 (Clone 2A3) , BD Biosciences , 340939 , Flow cytometry analysis: Surface protein staining.

Techniques: Adhesive, Flow Cytometry, Staining, Dissection, Sample Prep, Ointment, Sterility, Saline